上海信帆生物带您了解：组织自发荧光淬灭剂 

上海信帆生物有限公司 供应：组织自发荧光淬灭剂 ，产品有大量现货，质量稳定，提供质检报告，提供中英文说明书，欢迎咨询！

组织机构自行荧光淬灭剂 

表述：有很多集体结构会引起可穿透各样可见光波长滤光片的集体结构内源性组织化性荧光，同质性侵扰表面抗原符号荧光查看以及导至荧光组化脱色出现未知错误。组织化性荧光淬灭采血管中的化合物需用接触策略获取组织化性荧光线光源分子式发出了的光学，影响该光学从激发起态重返基态并防止能源降低，故而淬灭自发性荧光。采用了seo的孵育时刻，可以大局限性地去除自愿荧光而不很深印象抗体阳性标记符号的荧光。

常用：繁多进行、细胞膜免疫抗体荧光染色法的组织化荧光清理。专门可用于中枢神经团队组织荧光淬灭。

儲存：4 ºC背光。

使用方法：

以下的环节在免疫性荧光组化上色完成后以后(而不是在荧光印染再次很久)执行程序。对相应的组织结构和神经元型号，有必须系统优化孵育日期方便大容许淬灭自行荧光而不看不出关系免疫抗体标示的荧光(步奏2)。精心读书后原因分析。

1. 吸去PBS或某些洗衣加载液，用分馏水暂时清洗阴道组织结构薄片或组织细胞系培养教育板中的组织细胞系。

2. 下载通常但足够的的自愿荧光淬灭剂复盖团队切开或瓶皿中的组织。恒温10-90min。

3. 吸去自行荧光淬灭剂，用萃取水瞬间擦洗。

4. 吸去减压去离子水，用PBS遍布阻止切成片或是在肿瘤细胞系养成板中的肿瘤细胞系。

5. 封片。提倡采用抗荧光衰减封糖衣片。该封糖衣片可以免抵抗能力标签荧光低迷。

6. 荧光显微镜看。

就说明：

1.差异外来物种差异性质的结构的自行荧光都具有差异的表现形式，用到结构自愿荧光淬灭剂的疗效机会就会有相差。还有就是，所有对於自愿荧光的淬灭，早已在相应层面上影响抗体阳性阳性荧光功效。所幸的是该采血管对自愿荧光的淬灭层面不远不近超出范围抗体阳性阳性荧光功效的影响，以致能在矛盾律左右获得了非常好的平衡点。是因为不很清析的其原因，本采血管消失脑脊髓运动神经组识的自愿荧光具备着更加好的疗效。

2. 为赚取佳治疗效果，都要提升孵育时间间隔便于大可能淬灭对某类某一阻止的组织化荧光而不严重印象抗体阳性标记符号的荧光(操作步骤2)。至关重要的生物标本应在确定好佳孵育耗时时候运用本化学制剂。实施整合时，能取数张阻止性切开或靠近培植皿中的神经元，在免疫系统荧光组化染色法完后时候加进阻止性自愿荧光淬灭剂，孵育5、10、30、60、90分鐘等各种时光，冲洗器后观察动物荧光。但假如组建自发性荧光仍旧好强，可不断增加孵育时光；但假如孵育时光不大于10分钟的英文而荧光褪去十分的显著的，可将孵育精力下降为1-5半小时，可能可以采取出多量的组织机构组织化荧光淬灭剂加等份的双蒸水溶解，以后孵育10-90分调优。

3. 阻止组织化荧光淬灭剂应该在成功免疫检测荧光组化印染后利用，以免将嚴重有效降低免疫抗体荧光。

AutoFluo Quencher

Description:

Many tissues can produce endogenous spontaneous fluorescence which can be observed through various wavelength filters, which can obviously interfere with antibody labeling fluorescence and even lead to the failure of fluorescence histochemical staining. The ions in the spontaneous fluorescence quenching reagent can capture the electrons emitted by the spontaneous fluorescent light source molecules in a collision mode, which prevents the electron from returning to the ground state and prevents the energy release from the excited state, thereby quenching the spontaneous fluorescence. By optimizing the incubation time, the fluorescence of the antibody could be eliminated and the fluorescence was not obviously affected by the spontaneous fluorescence. 

Application: spontaneous fluorescence elimination of various tissues and cells by immunofluorescence staining. In particular, the application of neural tissue spontaneous fluorescence quenching.

Storage at :  4 degrees C & keep away from light.

Usage:

The following steps are performed after the IHC staining (and not before the fluorescent staining). For specific tissues and cell types, it is necessary to optimize the incubation time in order to maximize the quenching of the fluorescence (step 2), which is not significantly affected by the antibody labeling. Read the instructions carefully.

1 suction to PBS or the corresponding washing buffer, using distilled water briefly washed tissue sections or cell culture plate in the cell.

2 adding adequate amount of spontaneous fluorescent quenching agent to cover the cells in tissue sections or bottles. Room temperature 10-90min.

3 to absorb the spontaneous fluorescence quenching agent, with distilled water briefly rinse.

4 suck the distilled water, cover the tissue sections with PBS or cells in the cell culture plate.

5 mounting. Anti sealing agents using the proposed fluorescence decay. The sealing agents can prevent the antibody labeled with fluorescent decay.

6 fluorescence microscope observation.

Note：

1. different types of tissues of different types of spontaneous fluorescence with different characteristics, the use of spontaneous fluorescence quenching agent effect may be different. In addition, any quenching of spontaneous fluorescence will reduce the fluorescence intensity of antibody to a certain extent. Fortunately, the quenching degree of the reagent is far more than the decrease of the fluorescence intensity of the antibody, so that a good balance between the two can be obtained. Because of the unclear reasons, this reagent eliminates the spontaneous fluorescence of the spinal cord nerve tissue and has a better effect.

2. in order to obtain the best results, it is necessary to optimize the incubation time in order to maximize the quenching of the spontaneous fluorescence of a particular tissue and not to affect the fluorescence (step 2). Important specimens should be used to determine the optimal incubation time. Optimize, the number of desirable tissue section or in the cells in a Petri dish, after immunohistochemistry after adding tissue fluorescence quenching agent, were incubated for 5, 10, 30, 60, 90 minutes of different time, observe the fluorescence after washing. If the tissue autofluorescence is still strong, can prolong the incubation time; if the incubation time is less than 10 minutes and fluorescence fade is very obvious, can reduce the incubation time for 1-5 minutes, or you can remove a small amount of tissue autofluorescence quenching agent with equal distilled water dilution, and then incubated for 10-90 minutes optimization.

3. tissue spontaneous fluorescent quenching agent must be used after the completion of immunohistochemical staining, otherwise it will seriously reduce the antibody fluorescence.

上海信帆生物带您了解：组织自发荧光淬灭剂