信帆生物：细胞因子类ELISA试剂盒文献上线

《P-selectin glycoprotein ligand 1 deficiency prevents development of acute pancreatitis by attenuating leukocyte infiltration》

英文论文：

BACKGROUND Acute pancreatitis(AP) is rapid-onset pancreatic inflammation that causes local and systemic inflammatory response syndrome(SIRS) with high morbidity and mortality, but no approved therapies are currently available. P-selectin glycoprotein ligand 1(PSGL-1) is a transmembrane glycoprotein to initiate inflammatory responses. We hypothesized that PSGL-1 may be involved in the development of AP and would be a new target for the treatment of AP.AIM To investigate the role and mechanism of PSGL-1 in the development of AP.METHODS The PSGL-1 expression on leukocytes was detected in peripheral blood of AP patients and volunteers. Pancreatic injury, inflammatory cytokines expression, and inflammatory cell infiltration was measured in AP mouse models induced with PSGL-1 knockout(PSGL-1-/-) and wild-type(PSGL-1+/+) mice. Leukocyteendothelial cell adhesion was measured in a peripheral blood mononuclear cell(PBMC)-endothelial cell coculture system.RESULTS The expression of PSGL-1 on monocytes and neutrophils was significantly increased in AP patients. 

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二、操作关键步骤关键步骤：

1. 从恒温动平衡20min后的铝箔纸袋中取掉想要板条，累计板条用自封袋密封带放回4℃。

2. 设备基准品孔和范例孔，基准品孔各加不同的溶液浓度的基准品50μL。

3. 模板孔里添加入待测模板50μL；空页孔没有。

4. 除空白页孔外，规则品孔和样例孔中每孔融入辣根过钝化物酶（HRP）标出的检测工具表面抗原100μL，用封板膜封好反响孔，37℃水浴锅或温控箱温育60min。

5. 弃去液，吸水能力一张纸拍干，每孔加完洗條液（350μL），静置1min，甩去洗衣液，吸潮A4纸拍干，一样再次洗板5次（也用于洗板机洗板）。

6. 每孔建立底物A、B各50μL，37℃遮光孵育15min。

7. 每孔放入暂停液50μL，15min内，在450nm光谱处检测法各孔的OD值。

三、在使用安会具体措施：

请尽量穿带好来进行来进行实验性服、个人安全防护安全防护面罩和来进行来进行实验性棉手套等，做来进行来进行实验性个人安全防护。坚决杜绝会直接接触性货品。乘余货品请不要任意扔掉，，请按照照来进行来进行实验性室相关联微生物和药剂学废料物的处置法律规定，来进行处置。

信帆生物：细胞因子类ELISA试剂盒文献上线