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Rabbit Anti-BNP
Catalog Number :HZ246732 Quantity size : 0.1ml （dilute with pH 7.4
0.01 M PBS or antibody diluent ）
Rabbit Anti-BNPBackground: Brain natriuretic peptide （BNP） circulates in blood as a peptide
hormone with natriuretic, vasodilatory and renin inhibitory properties. BNP is secreted
predominantly by the left ventricular myocytes in response to volume expansion and
pressure overload. BNP belongs to a family of structurally similar peptide hormones,
which includes atrial natriuretic peptide （ANP）, BNP, C-type natriuretic peptide （CNP）
and urodilatin. These peptides are characterized by a common 17 amino acid ring
structure with a disulfide bond between two cystein residues. This ring structure
shows high homology between different natriuretic peptides （eleven of the 17 amino
acid residues are homologous in the ring of each of the natriuretic peptides）. BNP is a
32 amino acid peptide with disulfide bond between the cysteine residues Cys10 and
Cys26. In earlier studies it has been demonstrated that BNP concentration in blood
increases with the severity of congestive heart failure. Quantitative measurement of
BNP in blood provides an objective indicator of congestive heart failure severity.
specificity :
– Anti- BNP is a rabbit polyclonal antibody unconjugated
– specific for BNP of human, mouse and rat
– use for western blotting,elisa,immunoprecipitation and immunohistochemistry
– Protein G affinity chromatography purification, purity :>95%
– Isotype: IgG
– mol wt: 13kDa
Application :
– Western blotting 1:100-500
– Immunohistochemistry 1:100-500
– ELISA 1:500-1000
– Optimal working dilutions must be determined by the end user.
Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The
lyophilized antibody is stable at room temperature for at least one month and for
greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M
PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃
Important Note: This product as supplied is intended for research use only, not for
use in human, therapeutic or diagnostic applications.